-2] As far as "additive spec", consumers can and will put anything into their fuel tanks. Spec, is a "fuel additive designed or approved for use in the Makers product".
The Owners Manual of outboard engines typically describes what must be used in order to ensure that the warranty will be honored. I have not seen a manual that requires the use of a specific brand of fuel treatment or motor oil. The manuals I have seen lay out the specs that must be met, such as a motor oil that complies with the NMMA's FC-W for 4-stroke oil and TC-W3 for 2-stroke oils, but I've not yet seen any type of certification for a fuel treatment. There are not any such certifications out there. An OEM treatment would certainly be assumed to be designed for use in the manufacturer's engine while causing no harm, but that is not the same as saying that no others can be used. We strongly advise making sure of the contents of any fuel treatment being used.
-3] On a test conducted with your product quite some time ago, we mixed a small amount of water in a plastic container with some fresh fuel. We put in some Star-Tron in the correct doseage, and after agitation, the mixture separated forming a dark colored substance on the bottom of the container. Could you tell us what the separation consists of? In other words, in normal phase separation, the water and ethanol {as a percentage} will separate and fall to the bottom, leaving a degraded {octane deprived} fuel on top. This slurry is non-combustible an can clog filters, injectors, jets, etc. When I called Star-Tron, the answer I received was that this "slurry" is in fact combustible and can be safely burned through. My questions....what is the octane deprivation result from the leftover "fuel", and what "octane" does this now combustible slurry have? We will assume we started with 87....
Star Tron is not designed to be used in a test tube. Star Tron's enzymes act to disperse water thoughout the fuel in microscopic size so that it can be eliminated while the engine is operating in order to help avoid phase separation from occuring. There is nowhere for the water to go in a test tube, so that's not a valid experiment, as Star Tron is not an emulsifier (it's actually a de-emulsifier). If you add alcohol or any other type of emulsifying agent to water and fuel, the emulsifiers will "entrap/enjoin" the water, making it seem to go away. In reality, the water is still there. Emulsification actually degrades fuel quality, a fact borne out by the FAA and DOD's ban on emulsifying agents in aviation fuel, where that are classified as contaminants. It's a neat trick, but misleading, as the "disappearing water" is still there, but now in even larger clumps that will lead to degraded engine performance and the creation of carbon deposits. Adding more alcohol to E10 is not a great idea. As far as the dark slurry Bob saw, without seeing photos or knowing how much water was added, I cannot provide an answer. We've run a lot of tests but have never seen a reaction like that. Star Tron cannot make fuel suddenly go bad.
Phase separation takes place when the water content of E10 exceeds .5%, which is not all that typical a scenario. The normal issues attributable to E10 fuel are related to the formation of gum and other debris that occurs withing 30-45 days of the ethanol being blended with the gas. E10 is formulated to be used within 30 days.
If phase separation were to take place, the result would be a layer of gasoline under which you'd have the ethanol/water mixture (ethanol and water form a tight chemical bond, whiel ethanol adn gasoline do not form a chemcial bond). The gasoline would certainly have lost a few octane points, but there is no hard and fast answer as to what that amount would be.